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Development of an immunoassay for the detection of mycotoxins using xMAP technology and its evaluation in black tea samples (Record no. 129904)
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| fixed length control field | 02058nam a22001697a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 241025b |||||||| |||| 00| 0 eng dhttp://sndtjkoha.ourlib.in/cgi-bin/koha/cataloguing/addbiblio.pl# |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ishrat Jahan Saifi |
| 245 ## - TITLE STATEMENT | |
| Title | Development of an immunoassay for the detection of mycotoxins using xMAP technology and its evaluation in black tea samples |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p385-396 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. biblio.abstract | Mycotoxins, a natural food contaminant, are secondary metabolites of fungi. Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are two major mycotoxins found in various food commodities. These mycotoxins are hepatotoxic, nephrotoxic, cytotoxic, mutagenic and carcinogenic, thus they are a public health concern and their monitoring in food commodities is necessary. There are several conventional techniques available for mycotoxin detection, such as HPLC, LCMS, and ELISA. However, extensive nature and huge cost allowances make it challenging to deploy these techniques for monitoring of mycotoxins in the large sample size. Therefore, a robust, responsive and high-throughput technique is required. Here, we aimed to develop a multiplexed Luminex suspension assay based on multi analyte profiling (xMAP) technology for the simultaneous detection of AFB1 and OTA in the black tea, which is found to be contaminated with these mycotoxins during the cultivation or processing steps. Limit of detection for AFB1 and OTA, was 0.06 ng/ml and 0.49 ng/ml, respectively without any cross-reactivity with other mycotoxins and this assay is suitable for simultaneous detection of AFB1 and OTA in the same sample. Collectively, based on the results, we suggest that the developed Luminex suspension assay is sensitive, accurate, rapid and suitable for high-throughput screening of multiple mycotoxins. |
| 654 ## - SUBJECT ADDED ENTRY--FACETED TOPICAL TERMS | |
| Subject | <a href="Mycotoxins ">Mycotoxins </a> |
| -- | <a href="Aflatoxin B1 ">Aflatoxin B1 </a> |
| -- | <a href="Ochratoxin A">Ochratoxin A</a> |
| -- | <a href=" xMAP technology "> xMAP technology </a> |
| -- | <a href="Black tea">Black tea</a> |
| 773 0# - HOST ITEM ENTRY | |
| Host Biblionumber | 80310 |
| Host Itemnumber | 110087 |
| Place, publisher, and date of publication | Germany Springer |
| Title | Journal of Food Science and Technology |
| International Standard Serial Number | 0022-1155 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| 773 0# - HOST ITEM ENTRY | |
| -- | JP354 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| -- | ddc |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Location (home branch) | Sublocation or collection (holding branch) | Date acquired | Koha issues (times borrowed) | Piece designation (barcode) | Koha date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | SNDT Juhu | SNDT Juhu | 25/10/2024 | JP354.17 | 25/10/2024 | 25/10/2024 | Journal Article |