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Stability Indicating Method Development and Validation for the Simultaneous Estimation of Azilsartan and Chlorthalidone in their Bulk and Pharmaceutical Dosage Forms by RP-UPLC (Record no. 130114)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02384nam a2200181 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 241111b |||||||| |||| 00| 0 eng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Upender Rao Eslawath1 |
| 245 ## - TITLE STATEMENT | |
| Title | Stability Indicating Method Development and Validation for the Simultaneous Estimation of Azilsartan and Chlorthalidone in their Bulk and Pharmaceutical Dosage Forms by RP-UPLC |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P297-304 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. biblio.abstract | The present study is aimed towards developing and validating novel RP-UPLC analytical method for the analysis of Azilsartan and Chlorthalidone in their pharmaceutical formulations and to carry out stability studies by forced degradation to ascertain the stability of selected drugs. The proposed method was challenging as the method used in the study is novel for the estimation of antihypertensive drugs. Materials and Methods: The solubility studies of Azilsartan and Chlorthalidone were carried out at 25°C using various solvents and buffers. The solubility studies revealed that the selected drugs were soluble in methanol, water and acetonitrile. Hence, a mobile phase system consisting of Acetonitrile: Water: Methanol in the ratio of 20: 40: 40 v/v/v is used. The analysis of the drugs is accomplished using Phenomenex C18 column (4.5 µm, 250 × 2.2 mm ID) at 258 nm. Results and Discussion: The method developed is then validated according to the guidelines of ICH. The analysis results reveal that, method developed is within the acceptance limits for all the validated parameters. The forced degradation studies revealed that 9.9% of Azilsartan degradation is observed in peroxide presence and 5.6% of Chlorthalidone degradation is observed in the presence of acid solution. Summary and Conclusion: From the results of the study for the selected drugs, the sample recoveries in all the formulations were within the limits, hence the proposed method can be conveniently and easily adopted to the determination of Chlorthalidone and Azilsartan in combined formulation. |
| 654 ## - SUBJECT ADDED ENTRY--FACETED TOPICAL TERMS | |
| Subject | <a href=" Liquid Chromatography"> Liquid Chromatography</a> |
| -- | <a href=" Azilsartan,"> Azilsartan,</a> |
| -- | <a href=" Forced degradation,"> Forced degradation,</a> |
| -- | <a href=" Validation"> Validation</a> |
| -- | <a href=" Chlorthalidone."> Chlorthalidone.</a> |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kannappan N |
| -- | enkateshwarlu L2 |
| -- | Anton Smith A1 |
| 773 0# - HOST ITEM ENTRY | |
| Host Biblionumber | 125266 |
| Host Itemnumber | 109601 |
| Place, publisher, and date of publication | Banglore Association of Pharmaceutical Tearchers of India |
| Title | Indian Journal of Pharmaceutical Education and Research |
| International Standard Serial Number | 0019-5464 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| 773 0# - HOST ITEM ENTRY | |
| -- | JP44 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| -- | ddc |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Location (home branch) | Sublocation or collection (holding branch) | Date acquired | Koha issues (times borrowed) | Piece designation (barcode) | Koha date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | SNDT Juhu | SNDT Juhu | 11/11/2024 | JP44.31 | 11/11/2024 | 11/11/2024 | Journal Article |