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OPTIMIZATION OF α-AMYLASE SYNTHESIS BY BACILLUS VELEZENSIS USING TAGUCHI EXPERIMENTAL DESIGN (Record no. 131182)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01846nam a2200157 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250220b |||||||| |||| 00| 0 eng d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Item number | P.32-44 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Sasidhar Bhimana |
| 245 ## - TITLE STATEMENT | |
| Title | OPTIMIZATION OF α-AMYLASE SYNTHESIS BY BACILLUS VELEZENSIS USING TAGUCHI EXPERIMENTAL DESIGN |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P.32-44 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. biblio.abstract | This research employs the Taguchi experimental design to optimize the production of α-amylase, a versatile enzyme with diverse industrial applications, using Bacillus velezensis. Thirteen key parameters, including pH, temperature, agitation, inoculum size, aeration and various carbon and nitrogen sources, were systematically investigated using the Taguchi L27 (313) orthogonal array. Initial screening of six seed media formulations identified SM6 (0.5% peptone, 0.5% yeast extract, 0.1% K2 HPO4 ) as the optimal medium, producing 2.8 U mL-1 of α-amylase. Further optimization under the Taguchi framework yielded a maximum α-amylase activity of 1097.31 U mL-1 and total protein of 1230 mg mL-1 at pH 5, 34°C, 4% moong husk as carbon source, and 2% soybean cake as nitrogen source. Two-factor interaction analysis revealed that a temperature of 34°C with 2% soybean cake, and 4% moong husk with 2% soybean cake, provided the best results. Validation experiments confirmed a 31.2% enhancement in α-amylase production under the optimized conditions. This study highlights the efficacy of the Taguchi design in systematically optimizing complex bioprocesses, paving the way for improved α-amylase synthesis with B. velezensis. |
| 654 ## - SUBJECT ADDED ENTRY--FACETED TOPICAL TERMS | |
| Subject | <a href="a-amylase ">a-amylase </a> |
| -- | <a href="Bacillus velezensis ">Bacillus velezensis </a> |
| -- | <a href="Optimization ">Optimization </a> |
| -- | <a href="Taguchi design ">Taguchi design </a> |
| -- | <a href="Interaction analysis ">Interaction analysis </a> |
| -- | <a href="Bioprocess ">Bioprocess </a> |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saravanan Ravindran |
| 773 0# - HOST ITEM ENTRY | |
| Host Biblionumber | 125265 |
| Host Itemnumber | 109707 |
| Place, publisher, and date of publication | Mumbai Indian Drugs Manufacturer's Association |
| Title | Indian Drugs |
| International Standard Serial Number | 0019-462X |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Koha normalized classification for sorting | Not for loan | Location (home branch) | Sublocation or collection (holding branch) | Date acquired | Koha issues (times borrowed) | Koha full call number | Piece designation (barcode) | Koha date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | P__3244 | SNDT Juhu | SNDT Juhu | 20/02/2025 | P.32-44 | JP151.3 | 20/02/2025 | 20/02/2025 | Journal Article |