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Partially Purified Polysaccharides from Lentinus edodes (Mushroom) Scavenge Free Radicals and Induce Apoptosis in MCF-7 Cancer Cells by Regulating Apoptotic Genes (Record no. 131238)
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| fixed length control field | 02358nam a2200133 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250228b |||||||| |||| 00| 0 eng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Chandrasekaran Ponnusamy |
| 245 ## - TITLE STATEMENT | |
| Title | Partially Purified Polysaccharides from Lentinus edodes (Mushroom) Scavenge Free Radicals and Induce Apoptosis in MCF-7 Cancer Cells by Regulating Apoptotic Genes |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p535-545 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. biblio.abstract | Background: Therapeutic products obtained from nature have been gaining attention for health improvement, disease treatment, and drug development. Traditional therapeutic compounds from plants, animals, and microorganisms and their bioactive constituents have led to the development of therapeutics for various diseases, including cancer. Mushrooms are widely consumed products because of their nutritional and therapeutic potential. Materials and Methods: This study aimed to extract partially purified polysaccharides from Lentinus edodes (PLE) to a study to identify bioactive compounds by GC-MS and HPLC. Furthermore, we examined PLE to identify its antioxidant potential and effect on breast cancer cells (MCF-7 cell lines). Results: GC-MS revealed the presence of 45 bioactive components from PLE, and HPLC analysis showed the presence of β-glucan. Furthermore, PLE showed potential antioxidant activity, which was confirmed by significant free radical (DPPH, FRAP, and ABTS) scavenging and total antioxidant activity analysis. In addition, PLE showed noteworthy anticancer activity, substantiated by decreased cell viability in a dose-dependent manner (100, 200, 300, and 400μg/ mL) manner. On the other hand, RT-PCR analysis revealed that PLE potentially up-regulated caspase-3, toll-like receptor 4, and Bcl-2-associated X protein and concurrently down-regulated B-cell lymphoma 2 (Bcl-2), which confirmed that PLE can induce apoptosis in MCF-7 cancer cells. Conclusion: Hence, this study revealed that PLE can induce apoptosis in cancer cells and fight against breast cancer through its antioxidant potential. These results recommend that PLE might be considered a nutraceutical for managing breast cancer |
| 654 ## - SUBJECT ADDED ENTRY--FACETED TOPICAL TERMS | |
| Subject | <a href="Mushrooms, ">Mushrooms, </a> |
| -- | <a href="Nutraceuticals, ">Nutraceuticals, </a> |
| -- | <a href="Breast Cancer,">Breast Cancer,</a> |
| -- | <a href=" Apoptosis, "> Apoptosis, </a> |
| -- | <a href="Antioxidants.">Antioxidants.</a> |
| 773 0# - HOST ITEM ENTRY | |
| Host Biblionumber | 125266 |
| Host Itemnumber | 109790 |
| Place, publisher, and date of publication | Banglore Association of Pharmaceutical Tearchers of India |
| Title | Indian Journal of Pharmaceutical Education and Research |
| International Standard Serial Number | 0019-5464 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Location (home branch) | Sublocation or collection (holding branch) | Date acquired | Koha issues (times borrowed) | Piece designation (barcode) | Koha date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | SNDT Juhu | SNDT Juhu | 28/02/2025 | JP225.22 | 28/02/2025 | 28/02/2025 | Journal Article |