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Insecticidal activity of leaf proteins isolated from tuberous Ipomoea mauritiana against sweetpotato weevil (Record no. 131818)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02448nam a2200133 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250624b |||||||| |||| 00| 0 eng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | B. G. Sangeetha |
| 245 ## - TITLE STATEMENT | |
| Title | Insecticidal activity of leaf proteins isolated from tuberous Ipomoea mauritiana against sweetpotato weevil |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P 472-478 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. biblio.abstract | Sweetpotato is one of the most important food crops in<br/>the world and serves as a rich source of carbohydrates,<br/>betacarotene, vitamins and minerals. Sweetpotato<br/>weevil is one of the harmful pests infesting the crop<br/>and remains a challenge for crop cultivation. From<br/>screening of different Ipomoea spp., wild tuberous Ipomoea mauritiana was identified as resistant to sweetpotato weevil infestation. Hence, several extraction<br/>methods for the isolation of proteins from the leaves of<br/>different wild I. mauritiana, Ipomoea palmata, Ipomoea<br/>triloba and Ipomoea obscura were standardised. Further, the crude protein extract from I. mauritiana was<br/>tested against adult sweetpotato weevils using the leaf<br/>dip method. The mortality of the weevils were observed<br/>for crude extract isolated after 48 h at a concentration<br/>of protein ranges from 4 to 5 mg/ml. Further, to study<br/>the effects of crude protein of I. mauriatiana, the protein content and various enzymes from sweetpotato<br/>weevil were estimated after treatment. A significant<br/>reduction in the amount of protein was observed after<br/>treatment with crude leaf protein, which shows the<br/>presence of protease inhibitor activity. Significant difference was observed in the different enzyme activity,<br/>viz. superoxide dismutase, lipid peroxidase, glutathione<br/>peroxidase, glutathione reductase, glutathione s-transferase of sweetpotato weevil, which also reveals the<br/>insecticidal activity of crude protein extract. In conclusion, this is one of the first studies regarding the evaluation of insecticidal activity of leaf protein extract<br/>from the wild I. mauritiana against sweetpotato weevils, and our studies revealed the presence of insecticidal proteins as well as protease inhibitors from the<br/>leaves of I. mauritiana. Further studies will be done to<br/>identify and purify novel proteins from I. mauritiana<br/>using a proteomics approach to evaluate insecticidal<br/>activity against sweetpotato weevil. |
| 654 ## - SUBJECT ADDED ENTRY--FACETED TOPICAL TERMS | |
| Subject | <a href="Extraction">Extraction</a> |
| -- | <a href="protease inhibitory assay">protease inhibitory assay</a> |
| -- | <a href=" Ipomoea sp."> Ipomoea sp.</a> |
| -- | <a href="TCA-acetone">TCA-acetone</a> |
| -- | <a href="sweetpotato">sweetpotato</a> |
| -- | <a href="protein">protein</a> |
| 773 0# - HOST ITEM ENTRY | |
| Host Biblionumber | 125299 |
| Host Itemnumber | 112522 |
| Title | Current Science |
| International Standard Serial Number | 0011-3891 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Location (home branch) | Sublocation or collection (holding branch) | Date acquired | Koha issues (times borrowed) | Piece designation (barcode) | Koha date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | SNDT Juhu | SNDT Juhu | 24/06/2025 | JP670.5 | 24/06/2025 | 24/06/2025 | Journal Article |