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Foam index as a surrogate measure of protein content: an exploratory and inexpensive laboratory-free tool for students (Record no. 131824)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01985nam a2200133 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250624b |||||||| |||| 00| 0 eng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Rakshitha Madamakki |
| 245 ## - TITLE STATEMENT | |
| Title | Foam index as a surrogate measure of protein content: an exploratory and inexpensive laboratory-free tool for students |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P 495-504 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. biblio.abstract | An easily executable method to track nutritive potential of raw food grains at home is described here.<br/>Blanching the soaked grains with the boiled supernatant aided the release of protein into the supernatant<br/>which in-turn correlated with the protein content of<br/>the grains analysed in this study. When agitated, the<br/>supernatant generated stable froth, measured as foam<br/>index (FI). When compared with standard protein<br/>estimation methodologies (ultra-violet absorption and<br/>Folin–Lowry), both the percentage volume of the water<br/>absorbed (VA) and FI emerged as potential quantitative parameters. Correlation analyses using multiple<br/>grains of varying protein content indicated that VA<br/>and FI could serve as laboratory-free surrogate<br/>measures of protein content, and therefore a reflection<br/>of their nutritional value. To eliminate the possibility<br/>of surfactant-mediated foam, an ink-based ‘dye diffusion test’ was developed, wherein the dye was significantly retarded by protein foam. The simplicity of<br/>these assays were tested as a part of learning modules,<br/>successfully executed even by school students (12–15<br/>years old). Qualitatively, it engaged them in more authentic scientific inquiry in a way that was meaningfully associated with their daily lives. We propose that<br/>this technique creates opportunities for participation<br/>in citizen science, and can help address questions about<br/>cultivar growth in different climatic conditions when<br/>analysed temporally in a large networked format. |
| 654 ## - SUBJECT ADDED ENTRY--FACETED TOPICAL TERMS | |
| Subject | <a href="Froth, grains">Froth, grains</a> |
| -- | <a href="protein estimation">protein estimation</a> |
| -- | <a href="home lab">home lab</a> |
| -- | <a href=" nutrition"> nutrition</a> |
| 773 0# - HOST ITEM ENTRY | |
| Host Biblionumber | 125299 |
| Host Itemnumber | 112522 |
| Title | Current Science |
| International Standard Serial Number | 0011-3891 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Location (home branch) | Sublocation or collection (holding branch) | Date acquired | Koha issues (times borrowed) | Piece designation (barcode) | Koha date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | SNDT Juhu | SNDT Juhu | 24/06/2025 | JP670.8 | 24/06/2025 | 24/06/2025 | Journal Article |