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QUANTIFICATION OF PYRAZINAMIDE IN HUMAN PLASMA: DEVELOPMENT AND VALIDATION OF A LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY METHOD (Record no. 132973)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01699nam a2200145 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 251003b |||||||| |||| 00| 0 eng d |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Erica Alves |
| 245 ## - TITLE STATEMENT | |
| Title | QUANTIFICATION OF PYRAZINAMIDE IN HUMAN PLASMA: DEVELOPMENT AND VALIDATION OF A LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY METHOD |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | Page No. 47-55 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. biblio.abstract | A simple and efficient liquid chromatography-tandem mass spectrometry method was developed to quantify pyrazinamide in human plasma, using pyrazinamide D3 as the internal standard. Plasma samples were extracted with Hi-purity MCX cartridges via solid phase extraction. Chromatographic separation of pyrazinamide and the internal standard was performed on a Hypurity C8 column (100×4.6mm, 5µ) using isocratic conditions at 30 o C. The mobile phase consisted of an organic mixture and 10 mM ammonium acetate (50:50 V/V) at a flow rate of 1 mL min-1. Pyrazinamide and the internal standard eluted at 1.44 and 1.43 minutes, respectively, within a 3-minute runtime. Detection employed the use of a Hypurity HPLC column coupled with an AB Sciex API 4000 tandem mass spectrometer with electrospray ionization. Pyrazinamide was detected via ion transition 124.2 to 81, while the IS used ion transition 127.2 to 84. The method was validated as per European Medicine Agency guidelines, meeting acceptance criteria. |
| 654 ## - SUBJECT ADDED ENTRY--FACETED TOPICAL TERMS | |
| Subject | <a href="Liquid chromatography tandem mass [LC-MS/MS]">Liquid chromatography tandem mass [LC-MS/MS]</a> |
| -- | <a href="Pyrazinamide ">Pyrazinamide </a> |
| -- | <a href="pyrazinamide D3">pyrazinamide D3</a> |
| -- | <a href="Solid phase extraction [SPE]">Solid phase extraction [SPE]</a> |
| -- | <a href="electrospray ionization european medicine agency [EMA]">electrospray ionization european medicine agency [EMA]</a> |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | , Celina Nazareth |
| 773 0# - HOST ITEM ENTRY | |
| Host Biblionumber | 125265 |
| Host Itemnumber | 114075 |
| Place, publisher, and date of publication | Mumbai Indian Drugs Manufacturer's Association |
| Title | Indian Drugs |
| International Standard Serial Number | 0019-462X |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Location (home branch) | Sublocation or collection (holding branch) | Date acquired | Koha issues (times borrowed) | Piece designation (barcode) | Koha date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | SNDT Juhu | SNDT Juhu | 03/10/2025 | JP942.4 | 03/10/2025 | 03/10/2025 | Journal Article |