The aim of the present work is to use the latest achievements of nanotechnology (atomic layer deposition, ALD) in the field of food packaging to prevent biofilm formation by food-associated bacteria. Some potential applications of nanotechnology in the food packaging industry are studied in the manuscript, in the field of antibacterial materials for food packaging. The ALD technique was used to synthesize vanadium (V)-doped TiO2 thin nanofilm on commercially available polypropylene (PP) food container to enhance an antibacterial activity for potential use in food packaging, to reduce spoilage, thereby, prolonging the food shelf- life. To better understand the ability and effectiveness of the antimicrobial packaging material of V-doped TiO2, to prevent the biofilm formation by dairy-associated pathogenic bacteria, the coated and uncoated PP containers with a fresh raw cow’s milk were tested. We have illustrated the effectiveness of ALD Al2O3 + TiVOx nanocoating against populations of milk-borne pathogenic bacteria.