TY  - BOOK
AU  - Kavitha D
AU  - Madhumitha M
TI  - Dynamic protective potential of Couroupitaguianensis Aubl. against reactive free radicals: An approach of simulated in vitro model
KW  - Antioxidant
KW  - Couroupita guianensis Aubl
KW  - Free radicals
KW  - Goat liver slices
KW  - In vitro mode
N2  - Oxygen is a prominent element in the well-being of humans, but also responsible for causing tissue damage. The oxygen molecule gets reduced to form a highly reactive oxygen species due to various metabolic events happening in the host. The generated free radicals could react with other biomolecules like protein, lipid, carbohydrate and nucleic acid to impart severediseases and disorders. Antioxidants could effectively block the oxidation process by neutralizing the produced free radicals. Hence,  the  present  study  aims  to  validate  the  potent  antioxidant  activities  of  Couroupita  guianensis  Aubl.  using  goat  liver  slices  that  could  serve  as  an  in  vivo  simulated in  vitro  model.  The  methanolic  extract  and  the  alkaloid  fraction  of  both  leaves  and  flowers  of  C.  guianensis  Aubl.  were  prepared  and  their  enzymatic  and  non-enzymatic  antioxidant  activities  were  evaluated.  Hydrogen  peroxide  was  used  as  a  standard  free  radical  throughout  the  experiments  in  all  the  treatment  groups.  The  results  have  envisaged  that  the  plant  has  enormous  antioxidant  activities  in  oxidatively  stressed  goat  liver  slices  to  combat  various  oxidative  stress-related  diseases  and  disorders.  Conclusively,  the  in  vitro  model system to explore the antioxidant profiles will eventually mitigate the sacrifice of animals by exactly mimicking the in vivo conditions for research endeavors
ER  -