TY  - BOOK
AU  - Saira Salim
AU  - Gopika Jayasenan
TI  - Evaluation of pharmacological properties and identification of biopotent compounds of the medicinal herb Justicia simplex D. Don
KW  - Anticancer
KW  - Antimicrobial
KW  - FTIR
KW  - HRMS
KW  -  Justicia simplex
KW  -  MTT assay
N1  - Innovative research is going on in search of novel, safer, and more effective drugs that are affordable to the common man. The present study evaluates the wound healing, antibacterial, antifungal and anticancer efficacies of leaves of the folk medicinal herb Justicia simplex, identifies the bioactive compounds present in the extract and predicts the drug likeness and pharmacological activities of the compounds. The wound healing efficacy of the extract was evaluated by in vitro scratch assay, antimicrobial activities by agar well diffusion method, antioxidant activity by DPPH radical scavenging assay, cytotoxic activity by MTT assay, morphological studies were conducted using phase contrast microscopy, and cell apoptosis by acridine orange and ethidium bromide double staining method. FTIR and HRMS analyses were employed to identify the bioactive compounds present in the extract. Physicochemical characters and drug-likeness of the compounds were predicted employing the SWISSADME web tool and pharmacological activities by the PASS online software.The study proved the effectiveness of J. simplex against pathogenic bacteria, fungi, and its wound healing, anticancer and cell apoptotic properties. The bioactive compounds identified were ethyl 6,9,12-hexadecatrienoate, 17-octadecynoic acid, n-hexadecanoic acid and ethylene glycol 0,0-di(pivaloyl). Pa > 0.7 indicates that all the compounds are very likely to exhibit anti-eczematic activity and three compounds obey the Lipinski rule of drug likeness. While the study highlights J. simplex as a promising candidate for novel drug discovery, further research is needed to fully explore and validate its therapeutic potential
ER  -