000			01966nam a22001697a 4500
003			OSt
005			20241026111355.0
008			241026b |||||||| |||| 00| 0 eng d
100			_aRitika Kaushal
245			_aValorization of pineapple rind for bromelain extraction using microwave assisted technique: optimization, purification, and structural characterization
300			_ap 551–562
520			_aHuge amount of waste is generated by the pineapple processing industries which raises concerns regarding its safe disposal into the environment. This ever-increasing problem of waste management can be solved by the valorization of pineapple by-products to high-value compounds. The extraction of proteolytic enzyme, bromelain from pineapple rind using green techniques can help to overcome the drawbacks associated with conventional methods. In the present study, the extraction of bromelain from pineapple rind using microwave assisted technique resulted in considerable amount of proteolytic activity (127.8 U/mL) and protein content (2.55 mg/mL). The optimized extraction conditions were found as 200 W microwave power, 1:5 solid/ liquid ratio and after treatment time of 10 min. Highest specific activity (512 U/mg) of bromelain was obtained after using gel filtration chromatography. FTIR result confirmed the presence of functional groups in bromelain, whereas, XRD analysis indicated the semi-crystalline nature of bromelain. The results indicated MAE as an effective green technique for the extraction of bromelain from pineapple rind. The proteolytic action of the extracted bromelain makes it a suitable functional ingredient for its applications in bakery, dairy, and seafood processing industries.
654			_aBromelain _aPineapple rind _aMicrowave assisted extraction _a Valorization _aGreen techniques
773	0		_080310 _9110105 _dGermany Springer _oJP336 _tJournal of Food Science and Technology _x0022-1155
942			_cJA
942			_2ddc
999			_c129924 _d129924