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100			_aAhmed Reda Belmamoun1
245			_aChrysanthemum coronarium L: Chemical Composition and Gastroprotective Potential of Methanolic Leaf Extract in Ethanol-induced Gastric Ulcers in Male Wistar Rats
300			_aP-139-144
520			_aOur study evaluated the effect of Chrysanthemum coronarium L. (CC) leaves on ethanol-induced acute gastric ulcers in Wistar rats. Materials and Methods: The organic extract of CC was obtained by Soxhlet extraction with methanol and then divided into two dose groups: 250 mg/kg and 500 mg/kg. Omeprazole was used as a positive control at 20 mg/ kg. Our extract was subjected to the separation of bioactive compounds by High-Performance Liquid Chromatography (HPLC). Lethality tests (LD50) were carried out using standard procedures. Gastric protection was assessed by measuring gastric juice volume, total acidity, and free acidity. Gastric mucosal damage was assessed by histopathological examination. Results: Chromatographic analysis of the Methanolic Extract of Chrysanthemum coronarium L. (MECC) identified the presence of 19 phenolic compounds, representing 46.47% of the total sample. The dominant components were o-coumaric acid (9.55%), chlorogenic acid (6%), myricetin (4.19%), and benzoic acid (2.87%). Oral LD50 value was more than 5000 mg/kg in rat. In the present study, the methanolic extract of CC decreased total and free gastric acidity (53.80±7.038 and 17.8±2.375 respectively) for the 500 mg/kg dose and (60.40±4.490 and 24.8±1.855 respectively) for the 250 mg/kg dose. Omeprazole also decreased free and total gastric acidity (54.40±3.092 and 20±2.449 respectively), compared with the ethanol groups (19.40±2.909 and 4.4±0.678 respectively). In the histological study, we found that the gastric mucosal barrier could be significantly strengthened when the rats were pretreated with 500 mg/kg of the MECC and showed almost normal histology compared with the ethanol-ulcerated groups. Conclusion: Based on the present results, we can conclude that CC leaves could be a promising food for the protection of the gastric mucosa against ethanol-induced lesions
654			_a Chrysanthemum coronarium L _a Hplc, LD5 _a Ethanol, _a Gastric ulcer _aRat.
700			_aAbdelkader Ammam2
700			_a Chafik Mhamdia3
773	0		_0125266 _9109601 _dBanglore Association of Pharmaceutical Tearchers of India _oJP44 _tIndian Journal of Pharmaceutical Education and Research _x0019-5464
942			_cJA
942			_2ddc
999			_c130066 _d130066