000			02005nam a2200181 4500
003			OSt
005			20241218155209.0
008			241218b |||||||| |||| 00| 0 eng d
100			_aSrimathi Raghavan
245			_aInhibition of Bungarus caeruleus snake venom toxicity by Citrus reticulata methanolic extract and in-silico analysis of possible binding modes
300			_aP.67-76
520			_aSnakebite envenomation has been one of the major causes of morbidity and mortality around the world. Though intravenous administration of commercial anti-venom systemically neutralizes the toxicity, adverse effects are commonly seen in the post-treatment period. Plant metabolites and their derivatives have been shown to exhibit a wide range of anti-ophidian properties. In this study, we have investigated the Bungaruscaeruleus venom neutralization potential of Citrusaurantiumand Citrus reticulata Blanco peels in terms of phospholipase and hemolytic inhibition. Methanolic extract of C. reticulata exhibited the highest inhibition (93%) of PLA2 activity. However, the extracts of both these Citrusspecies significantly inhibited the hemolytic activity of the venom (54–82%). Molecular docking indicated the binding of thecitrus metabolites to catalytic site residues (TRP19, GLY30, TYR31, GLY32, and HIS48) of PLA2. In vivostudies using Swiss Albino mice confirmed the neutralising capacity of the citrus peel extract, as reflected by the increase in survival time. Therefore, the study reveals that these metabolites in the methanolic extract of the citrus peel can be used as supplements for the management of snake bites.
654			_a Snakebite _aCitrus aurantium _a Citrus reticulata _aVenom _a Neutralization _a Bungarus caeruleus
700			_aGurunathan Jayaraman
773	0		_0125286 _9109604 _dNew Delhi NISCAIR _oJP47 _tIndian Journal of Traditional Knowledge _x0972-5938
942			_cJA
942			_2ddc
999			_c130682 _d130682