000			01705nam a2200181 4500
003			OSt
005			20250114123302.0
008			250114b |||||||| |||| 00| 0 eng d
100			_aKalaiselvi Ponnusamy
245			_aNOVEL METHOD FOR DETERMINATION OF POMALIDOMIDE IN HUMAN PLASMA BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY / MASS SPECTROSCOPY
300			_aP.57-62
520			_aThe current investigation studied and verified an accurate Ultra performance liquid chromatography mass spectroscopy/ mass spectroscopy technique for the high-throughput detection of pomalidomide in human plasma with celecoxib as an internal standard. Chromatographic studies were carried out on Hypersil BDS (50 mm x 4.1 mm, 5m) with an isocratic mobile phase of acetonitrile: ammonium formate (80:20, V/V), at a flow rate of 0.5 mL min-1 and a total run time of 3 min. A triple quadruple Tandem Mass spectroscopy with electrospray ionization in positive mode was utilized to identify the analyte. Using multiple reaction monitoring modes and precursor-to-product ion transitions of 274.43 m/z for pomalidomide and 382.12 m/z for standard, respectively, pomalidomide and internal standard were measured. The developed technique was verified as per the regulatory standards for bioanalytical procedures validation. Other validation results also match.
654			_aPomalidomide _alower limit of quantification _aultra-performance liquid chromatography _acelecoxib _abioanalytical _amatrix stability
700			_aKathiravan Natarasan
773	0		_0125265 _9111177 _dMumbai Indian Drugs Manufacturer's Association _oJP490 _tIndian Drugs _x0019-462X
942			_cJA
942			_2ddc
999			_c130738 _d130738