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100			_aKrishna Desai
245			_aEvaluation of extraction techniques and solvents for phytochemical screening, antioxidant and antimicrobial activity of mungbean (Vigna radiata (L.) R. Wilczek) seeds
300			_ap. 565-577
520			_aThe mung bean (Vigna radiata (L.) R. Wilczek), belonging to Leguminoseae family, is major short-seasoned legume grown in tropical and subtropical regions, mainly during the Kharif and summer seasons. Present investigation aimed to check the effects of different extraction techniques and solvents on FTIR spectrophotometric analysis and quantitative profiling of phenols and flavonoids, followed by an assessment of the antioxidant and antimicrobial activity of mung bean seeds. Methanolic extracts of seeds obtained through Soxhlet extraction, maceration and ultrasonication were subjected to solvent-aided fractionation using the separation funnel method. The extractive yield of obtained fractions ranged from 2.86 to 59.85%. The results indicated maximum yield in aqueous fractions, followed by butanol and hexane fractions, while the lowest in ethyl acetate and chloroform fractions. The phenolic content, flavonoid content and FRAP values in the different fractions varied from 16.32±1.67 to 56.82±0.88 mg of GAE/g, 38.21±0.07 to 149.95±0.53 mg QE/g, and 41.54±0.56 to 104.81±0.48 mg Fe (II) E/g, respectively. Phenolics were higher in non-polar fractions, while flavonoids and FRAP values were higher in polar fractions. Pearson correlation displayed a strong correlation between TFC and FRAP assay. The antimicrobial activity revealed Staphylococcus aureus as the most susceptible, followed by Bacillus subtilis and Saccharomyces cerevisiae; conversely, Pseudomonas sp. was shown to be most resistant with no inhibition activity. Overall, ethyl acetate fractions from all the extraction techniques presented the best results against tested microbial samples. PCA analysis presented significant discrimination among solvents and extraction techniques. Mung beans can be used as nutraceuticals pharmaceuticals and functional foods.
654			_aAntimicrobial activity _aFractionation _aFTIR _aPhytocomponents _aPrincipal component analysis _aVigna radiate
700			_aNainesh R. Modi
773	0		_0125285 _9111204 _dNew Delhi NISCAIR _oJP501 _tIndian Journal of Natural Products and Resources _x0976-0504
942			_cJA
942			_2ddc
999			_c130827 _d130827