000			01649nam a2200133 4500
008			250624b |||||||| |||| 00| 0 eng d
100			_aAmandeep Singh
245			_aEpigenetic DNA methylation markers: a non-invasive diagnostic approach for urinary bladder cancer
300			_aP 398-403
520			_aUrinary bladder cancer (UBC) has high recurrence rates, making early detection vital. Promoter DNA methylation of CALCA, CCNA1 and P16 genes can serve as non-invasive biomarkers. The objective of the present study is to evaluate the promoter methylation status of CCNA1, CALCA and P16 genes in the blood of UBC patients compared to controls, and to explore their utility as non-invasive biomarkers. Blood samples from 169 participants, including 89 UBC patients and 80 controls, were analysed. Blood samples were collected, DNA was extracted for bisulphite conversion, followed by methylation-specific polymerase chain reaction (PCR). Statistical analyses determined the association between methylation status and clinical features. Methylation was detected in CCNA1 (66.2%), CALCA (53.9%), and P16 (72%) in UBC patients, significantly higher than in controls. Combined analysis of these three genes provided a sensitivity of 85% and specificity of 80% for UBC detection. Blood-based methylation of CCNA1, CALCA and P16 genes emerged as a promising non-invasive biomarker for early UBC detection. Further validation in larger cohorts is required.
654			_aDNA methylation _amethylation-specific PCR _anon-invasive biomarkers _aurinary bladder cancer
773	0		_0125299 _9112521 _tCurrent Science _x 0011-3891
942			_cJA
999			_c131777 _d131777