000			01821nam a2200145 4500
008			250718b |||||||| |||| 00| 0 eng d
100			_aAkhilesh P. Naik
245			_aPHYTOCHEMICALS FROM WITHANIA SOMNIFERA AS A NOVEL STRATEGY AGAINST ANTIBIOTIC RESISTANT PSEUDOMONAS AERUGINOSA
300			_app49-59
520			_aAntibiotic overuse has accelerated antimicrobial resistance (AMR), posing significant health and environmental risks. This study investigates the potential of Withania somnifera (Ashwagandha) in combating AMR in Pseudomonas aeruginosa, which exhibits resistance to certain aminoglycoside antibiotics. Disc diffusion assays across 14 antibiotics identified resistance exclusively to gentamicin. Phytochemicals from various parts of Ashwagandha were tested against resistant P. aeruginosa strains. Molecular docking of 68 phytochemicals against MexZ and aminoglycoside 3’-N-acetyltransferase proteins identified lead compounds, while molecular dynamics simulations of MexZ with withaferin A and withanolide B assessed the stability of protein-ligand complexes. The methanolic root extract proved most effective, producing a 34 mm inhibition zone. Molecular docking revealed binding affinities below –8.0 kcal mol-1 in multiple compounds, with key stability parameters from Molecular docking simulation supporting the potential for drug design. The findings highlight Ashwagandha root phytochemicals as promising candidates for overcoming antibiotic resistance in P. aeruginosa.
654			_aPseudomonas aeruginosa _awithania somnifera _aPhytochemicals _aMolecular docking _aMolecular dynamics simulation
700			_a Deeksha Prakasha
773	0		_0132135 _9113083 _aDhanashri S. Dhavale _tPHYTOCHEMICAL PROFILING AND GC-MS ANALYSIS OF AQUEOUS METHANOL FRACTION OF SEVYACHANDANADI LEPA
942			_cJA
999			_c132136 _d132136