000			01773nam a2200145 4500
008			250718b |||||||| |||| 00| 0 eng d
100			_aSumitra Pandaa
245			_aSOLVENT GUIDED EXTRACTS OF TWO OCIMUM SPECIES REVEALED VARIATIONS IN ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES
300			_app60-66
520			_aThe present study investigates the comparative antioxidant and antimicrobial potential in different solvent extracts of Ocimum sanctum and Ocimum basilicum collected from southeastern ghats highland agroclimatic zone of Odisha state, India. Extracts were prepared using solvents of increasing polarity, such as n-hexane, chloroform, ethyl acetate, methanol and water. DPPH (2,2-diphenyl-1-picrylhydrazyl) assay showed high antioxidant activity in methanolic extract of O. sanctum (IC50: 3.64 μg mL-1) and highest potency in ethyl acetate extract (IC50: 3.98 μg mL-1) of O. basilicum. Methanolic extracts of both species showed highest antimicrobial activity against Staphylococcus aureus and Escherichia coli (MIC: 6.25 μg mL-1, MBC: 12.5 μg mL-1). Methanolic extracts have highest phenolic and flavonoid content in O. basilicum (135.57 mg GAE g-1, 89.44 mg QE g-1) and O. sanctum (130.9 mg GAE g-1, 82.9 mg QE g-1). Solvent-guided extraction revealed significant variation in bioactivities, which could be analyzed in depth towards future improvement in drug discovery and as sustainable bioactive agents for therapeutic and industrial applications.
654			_aSequential extraction _aOcimum spp _aantioxidant activity _aTPC _aTFC _aAntimiceobial effect
700			_a Jyotirmayee Lenka
773	0		_0132135 _9113083 _aDhanashri S. Dhavale _tPHYTOCHEMICAL PROFILING AND GC-MS ANALYSIS OF AQUEOUS METHANOL FRACTION OF SEVYACHANDANADI LEPA
942			_cJA
999			_c132137 _d132137