SNDT WOMEN'S UNIVERSITY
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NOVEL METHOD FOR DETERMINATION OF POMALIDOMIDE IN HUMAN PLASMA BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY / MASS SPECTROSCOPY
Kalaiselvi Ponnusamy
NOVEL METHOD FOR DETERMINATION OF POMALIDOMIDE IN HUMAN PLASMA BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY / MASS SPECTROSCOPY - P.57-62
The current investigation studied and verified an accurate Ultra performance liquid chromatography mass spectroscopy/ mass spectroscopy technique for the high-throughput detection of pomalidomide in human plasma with celecoxib as an internal standard. Chromatographic studies were carried out on Hypersil BDS (50 mm x 4.1 mm, 5m) with an isocratic mobile phase of acetonitrile: ammonium formate (80:20, V/V), at a flow rate of 0.5 mL min-1 and a total run time of 3 min. A triple quadruple Tandem Mass spectroscopy with electrospray ionization in positive mode was utilized to identify the analyte. Using multiple reaction monitoring modes and precursor-to-product ion transitions of 274.43 m/z for pomalidomide and 382.12 m/z for standard, respectively, pomalidomide and internal standard were measured. The developed technique was verified as per the regulatory standards for bioanalytical procedures validation. Other validation results also match.
Pomalidomide
lower limit of quantification
ultra-performance liquid chromatography
celecoxib
bioanalytical
matrix stability
NOVEL METHOD FOR DETERMINATION OF POMALIDOMIDE IN HUMAN PLASMA BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY / MASS SPECTROSCOPY - P.57-62
The current investigation studied and verified an accurate Ultra performance liquid chromatography mass spectroscopy/ mass spectroscopy technique for the high-throughput detection of pomalidomide in human plasma with celecoxib as an internal standard. Chromatographic studies were carried out on Hypersil BDS (50 mm x 4.1 mm, 5m) with an isocratic mobile phase of acetonitrile: ammonium formate (80:20, V/V), at a flow rate of 0.5 mL min-1 and a total run time of 3 min. A triple quadruple Tandem Mass spectroscopy with electrospray ionization in positive mode was utilized to identify the analyte. Using multiple reaction monitoring modes and precursor-to-product ion transitions of 274.43 m/z for pomalidomide and 382.12 m/z for standard, respectively, pomalidomide and internal standard were measured. The developed technique was verified as per the regulatory standards for bioanalytical procedures validation. Other validation results also match.
Pomalidomide
lower limit of quantification
ultra-performance liquid chromatography
celecoxib
bioanalytical
matrix stability