Curcumin, a prominent polyphenol compound present in Curcumin longa L., exhibits diverse pharmacological and biological effects. Its hydrophobic nature and poor bioavailability necessitate its solubilization in an amphiphilic solvent such as dimethyl sulfoxide (DMSO) for research purpose. The present study aims to characterize a DMSO-solubilized curcumin and assess the angiogenesis potential of curcumin in zebrafish and chick chorioallantoic membrane (CAM) assay. Curcumin was characterized using UV-Vis spectrophotometry and the angiogenesis effect was evaluated using CAM and zebrafish embryos. The DMSO-solubilized curcumin’s UV-Vis spectrum revealed hypsochromatic shift in wavelength maximum from 425 to 347 nm, indicating solvent-curcumin interaction. Evaluation of angiogenesis activity in the CAM and zebrafish demonstrated dose-dependent promotion of angiogenesis. Curcumin’s biphasic, dose-dependent angiogenesis feature was validated in CAM and zebrafish models, affirming utility of zebrafish model for angiogenesis efficacy study. The solvent-curcumin interactions, molecular properties, and their therapeutic consequences need further investigation.