The present study aims to explore the possible association pattern of receptor activator of nuclear factor kappa B (RANK) gene variants (rs 35211496 and
rs 1805034) with its circulatory level and bone mineral density (BMD) in North Indian postmenopausal
women. In this case-control study, we recruited 165
postmenopausal osteoporotic women as cases (age
54.44 ± 6.00) and 165 healthy postmenopausal women
as controls (age 54.47 ± 6.46). BMDs were taken in
all subjects using dual energy X-ray absorptiometry
at different skeletal sites, and genotyping was carried
out using polymerase chain reaction and the restriction fragment length polymorphism method. RANK
serum levels were also assessed in all enrolled subjects by enzyme-linked immunosorbent assay. Findings from the present study indicated that subjects
with homozygous mutant TT genotype of rs35211496
(NC_000018.10 : g.62354528C > T) and rs1805034
(NC_000018.10 : g.62360008C > T) showed significantly
low BMD at forearm, lumbar spine, hip and femoral
neck and increased RANK level as compared to CC
and CT genotypes. Furthermore, at rs1805034, a
significant difference was noted in the frequency
of RANK genotypes and alleles among osteoporotic
cases and controls (P = 0.021; 0.005) respectively